Martin-Luther-Universität Halle-Wittenberg

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Teilprojekt B2

Funktionsanalyse des Elongatorkomplexes

Temporal and spatial control of cell growth and cell proliferation are of vital importance for plant development and adaptation. At the cellular level and along with its protein partner complexes, RNA polymerase II (pol II) holoenzyme serves to integrate both intra- and extracellular signals in order to coordinate such regulatory networks. At the heart of project B2 lies Elongator, a pol II associated complex, whose cellular function(s) are poorly understood. Elongator is a six-subunit protein complex that appears to be structurally conserved between yeast, plant and humans. Consistent with its transcriptional role as a pol II partner complex, Elongator has histone acetylase activity and interacts with nascent, unspliced mRNAs. Yeast Elongator has also recently been shown to be involved in tRNA modification and polarised exocytosis suggesting additional roles within the cell. Finally, Elongator effects the yeast killer toxin complex zymocin which is lethal for Saccharomyces cerevisiae cells. Toxicity requires Elongator interaction with protein Kti12/Tot4. Removal of Tot4 phenocopies yeast Elongator mutants and deletion of the TOT4 homologue DRL1 from Arabidopsis thaliana leads to deformed roots and leaves. Potentially, Tot4 and DRL1 may serve Elongator-related roles in yeast and plant, respectively. Elongator mutants cause pleiotropic phenotypes including slow growth and enhanced sensitivity to stresses in yeast as well as delayed flowering and organ underdevelopment in plants. The aim of project B2 is to identify Elongator from A. thaliana and to provide a detailed structure/function analysis by reconstituting the protein complex in S. cerevisiae and assaying cross-complementation. Further experimental work will focus on the role of the Elongator partner Tot4/DRL1 and how Elongator may be embedded in plant cellular signalling.

SonderFB 648B2

SonderFB 648B2

Ziele des Projektes:

  • Identifikation des pflanzlichen Elongators
  • Funktionsanalyse - Vergleich Hefe/Pflanze
  • Rolle von Elongatorregulator DRL1/Tot4
  • Einbindung in Signalkaskaden
  • Elongatorrelevanz für Pflanzenentwicklung (langfristig)

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